The matrix of most mitochondria had a coarse-fibered structure and high electron
density.
Tanks of a smooth endoplasmic reticulum had the form of vacuoles with electron-
transparent contents. A big number of membranes of a smooth endoplasmic reticulum
were subjected to fragmentation.
The granular endoplasmic reticulum membranes were also subjected to
fragmentation. On the membranes of the granular endoplasmic reticulum were detected
in a small number of ribosomes. Isolated free ribosomes and polysomes were located
in the electron-transparent cytoplasm of glandulocytes.
Destructive disorders develop in the submicroscopic organization Golgi complex
in the form of disorganization of smooth membranes, which acquired a chaotic
orientation under the influence of X-ray irradiation.
Large electron-transparent vacuoles were located next to them. Lipid inclusions
and secretory granules were located in the area of the localization of smooth
membranes of the lamellar cytoplasmic Golgi complex. There is a high electron density
of the cytoplasmic membrane (loosening, thickening, as well as foci of lysis).
CONCLUSION
The presence of dystrophic and destructive disorders of the ultrastructural
organization of "nurse" cells and interstitial endocrinocytes of rats’ testes after
exposure to X-ray irradiation was showed our electron microscopic study
The described mitochondrial changes in the form of loosening and focal lysis of
the external membranes and crista indicate that under the influence of X-ray irradiation
at the subcellular level mitochondrial dysfunction develops. This one entails a violation
of the bioenergetics supplies of synthetic and reparative intracellular processes.
Structural evidence of a decrease in the functional activity of these cells was a
decrease of ribosomes number, both linked to the membranes of the granular
endoplasmic reticulum, and to the ribosomes and polisomes that lie freely in the
cytoplasm, as well as focal destruction of the membranes, both granular and agranular
endoplasmic reticulum.
The reduction of the Golgi complex and the appearance of secondary lysosomes
and lipid inclusions in its area indicate the activation of catabolic processes in the
cytoplasm of Sertoli and Leydig cells.
BIBLIOGRAPHICAL REFERENCES
1. Protective effect of medroxyprogesterone acetate plus testosterone against
radiation-induced damage to the reproductive function of male rats and their offspring
/ Jégou B., Velez de la Calle J.F., Bauché F. // Proc Natl Acad Sci U S A. 1991 Oct
1;88(19):8710-4. https://www.ncbi.nlm.nih.gov/pubmed/1833765
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