form potassium ferrocyanide (Fe
2+
), which then reacts with ferric chloride to form
ferric ferrous complex that has an absotion maximum at 700 nm. To prepare the
reaction solution, a different amount of the extract, after the rotary evaporator, was
mixed (0.005 g, 0.01 g, 0.015 g, 0.02 g, 0.025 g) and dissolved in an appropriate solvent
(1 ml) and 1 ml phosphate buffer (0.2 M, pH 6.6) and 1 ml of potassium ferricyanide
solution (1%).The resulting solution was incubated at 50 for 20 minutes. Then 1ml of
trichloroacetic acid (10%) was added to terminate the reaction and was quenched under
running water for 5 minutes, the resulting mixture was centrifuged at 3000 rpm for 10
minutes. An aliquot of 2 ml was then removed from the top layer of each solution, to
which were added 2 ml of distilled water and 0.4 ml of ferric chloride solution
(0.1%).The solution absorbance was measured at 700 nm (10). Increasing absorbance
of the reaction mixture indicates increasing reducing power. Results were expressed as
a mean of standard deviation (SD) of 3 replicate measurements, with ascorbic acid as
a reference reducing agent. It was found that the reducing powers of extracts also
increased with the increase of their concentrations (Table 2). Reducing power is
associated with antioxidant activity and can serve as a significant reflection of
antioxidant activity.
Compounds with a reducing power show that they are electron donors that have
the ability to reduce oxidized intermediates of the processes of lipid peroxidation, they
can act as primary and secondary antioxidants. Higher absorbance of the reaction
mixture indicates higher reductive potential [9].
Table 2 Summary of various antioxidant capacities
Total antioxidant
activity
Reducing power
Concentration, mg/ml
107,72±4,2
0.5
1.0
1.5
2.0
2.5
0,10±0,04
0,21±0,07
0,36±0,04
1,42±0,11
2,03±0,09
Results were expressedby mean of standard deviation (SD) of three replicate
measurements with ascorbic acid as a reference reducing agent. The above studies,
during which the antioxidant activity of the extracts of the apricot pomace was
established, make it possible to recommend this plant as a means for correcting the
functional state of the free radical oxidation system. The chemical characterization of
aldehydes, quercetin, kaempferolin various studies has shown that these
compoundsexert various therapeutic effects, reduce oxidative stress and inhibitlipid
peroxidation of LDL, thereby preventing atherosclerosis[9]. Thus, a detailed study on
the identified compounds should be undertaken to determine the favourable actions of
these compounds on human health.
Based on the antioxidant activity exhibited by various plant extracts, many plants
with amino-containing compounds have been shown to be effective sources of
anticorrosion. Over recent years, the traditional approach on corrosion inhibitors has
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