experimental models. Diabetes was induced in rat’s males Vistar line (body weight 160
– 200 g) by using Streptozotocin in high dose of 70 mg/kg intraperitoneal.
Streptozotocin causes pancreatic beta-cell death by inducing apoptosis followed by
necrosis in rats, the acute hyperglycemia develops in 24–72 hours and continues in
prolonged time. High streptozotocin dose inducing diabetes model approximates to the
1 type human diabetes in hormonal and metabolic changes. The type 2 diabetes
modeling has been realized by introducing low dose of streptozotocin (40 mg/kg)
dissolved in the citrate buffer solution (pH 4.5). Before inducing diabetes, rats were
fed with high fat diet (daily ration included 40 % saturated fats) for 4 weeks; ad libitum
access to water, natural change of light regime, temperature and humidity had been
maintained according to the vivarium standards [7].
Antiexudative activity has been studied on carrageenan-induced paw edema in
rats. The 1 % carrageenan aqueous solution has been injected in dose 0.1 ml into back
right paw; the left paw was the control group. The volume of paw has been measured
with water oncometre [8]
.
The antialterative activity of the topical pharmaceutical composition based on
SAD has been studied using model of aseptic inflammation in rats (subcutaneous
injection of 0.5 ml of 9 % acetic acid solution with simultaneous intraperitoneal
application of 6 % dextran solution) a week after experimental diabetes had been
induced or without pathology.
The experimental animals have been divided into four groups. The first group has
been treated with test samples of SAD pharmaceutical composition on different
ointment bases; the second group has received placebo, the third group has been treated
with referent remedies (Diclofenac Sodium gel and ointment “Vundekhil”). The
control group included animals with 1 and 2 type diabetes and intact rats.
The studying of specific activity of the SAD pharmaceutical composition has been
carried out on carrageenan-induced paw edema and chemical trauma in rats (samples
№ 1-5, tab. 1) in the absence of diabetes. It has been proven that samples № 4-5 have
demonstrated significant antiexudative and antialterative effects. Sample № 5 has
intensively influenced pathological process, in comparison with other ointment
samples. Thus, strong inhibition of the exudative inflammation from 62.07 % to
74.42 % has been determined in 4-5 hours of the experiment, that was 1.5 –2 times
better result, than other groups demonstrated. Topical application of the samples № 4–
5 increased wound healing; complete healing has registered in 15-20 days from the
starting of the experiment. In comparative groups of rats the reparative processes
continued up to 30 days. Obtained positive results of the effectiveness in the diabetes
absence of the test sample's № 5 allow to recommend further investigations of its
activity under the condition of diabetes.
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