researchers in their works state that the essential acceleration of the extracting process
may be achieved already during the application of relatively slow mixing with the
fictive speed of the arm mixer endpoint 0.1 m/s, or during the extracting agent
circulation with its fictive speed in the apparatus of approximately 0.3 m/s [7]. Thus,
even such inessential hydrodynamic conditions release the external resistance during
the extraction process almost completely. However, there is one more prospective
group of factors influencing the mass-conductivity in the particles of raw material using
different physical phenomena. Such factors include: mechanical fluctuations, i.e.
vibrations, both high and low-frequency (ultrasound); hydraulic impacts of different
nature, electrodialysis [9]. The vacuum-filtration method of extracting shall be
separately mentioned as the process intensification method [6, 7].
Ultrasound as a physical factor of intensification of different technological
processes is used for more than half a century. Many researchers showed the suitability
of ultrasound for the extraction process intensification. However, no information was
found on the combination of the ultrasound disintegration and mechanical extraction
[7, 9].
To our point of view, the progressive method of the proteins and polysaccharides
obtainment from Сandidaalbіcans fungus biomass, is the combination of chemical and
physical methods, such as extraction with 5.0% solution of sodium hydroxide during
heating with constant mixing and extraction with 5.0 % sodium hydroxide with the
combination with ultrasound disintegration. Combination of physical and chemical
methods accelerates the diffusion and improves the permeability of the cell
membranes.
Studying the kinetics of extracting the biologically active substances from
Сandidaalbіcans fungus biomass under the influence of ultrasound was conducted
under the following parameters: Frequency of the ultrasound fluctuations 22 khz, the
ultrasound intensity - up to 10 W/cm2.
To determine the efficiency of the suggested methods of extracts obtainment it
was necessary to study the dynamics of extracting the researched substances from the
fungus biomass, both using the traditional extracting method and using the extraction
with 5.0% of the solution of sodium hydroxide in combination with the ultrasound
disintegration and extracting with 5.0% solution of sodium hydroxide in combination
with the intense mixing.
To prevent the contamination all manipulations were conducted in the laminar
box with the maintenance of aseptic conditions. The obtained dry biomass of
C.albicans fungus was milled, weighted 1 gr each and simultaneously the extraction
was conducted with 5.0% solution of sodium hydroxide at the temperature of 50 ± 2
ºС with constant mixing with the help of arm mixer with the rotation speed of 1000
r/min and with 5.0% of the solution of sodium hydroxide in combination with the
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