albicans fungus were studied on the animals with the simulated heterologous
generalized candidamycosis caused by the C. tropicalis fungus. The immuno-
diagnostics was conducted through one-time intradermal injection of the sterile fraction
to the infected animals in the amount of 0.1ml with the concentration of protein 5
µm/ml into the dehaired skin section on the 20th day after the infection.
According to the results of studies, the erythema is formed and in some cases the
papula in 24-48 hours. – the delayed-type reaction. According to the data obtained I
fraction of C. albicans fungus extract shows 20 % animals suffering from heterologous
candidamycosis caused by C. tropicalis fungus.
CONCLUSION
The theoretical and experimental grounding is provided of the approach as to the
development of the allergen for the immunodiagnostics of candida infection. Based on
the results of physical, chemical and biological studies, the technology of allergen was
elaborated for the immunodiagnostics of candida infection obtained from the biomass
of C.albicans fungus, composed of the protein and polysaccharides.
For the first time the theoretical and experimental grounding is provided of the
practicability of combining the physical and chemical methods of obtainment of the
allergic components of C.albicans fungus through the extraction with 5.0% solution of
sodium hydroxide with the ultrasound.
According to the results of the biochemical research, the composition of obtained
C. Albicans fungus extract was determined which contains the proteins,
polysaccharides and nucleic acids. The monosaccharide composition of
polysaccharides was determined, which contains mannose, glucose, and tracks of
ribose.
The grounding is provided of the technology of purification of C. albicans fungus
extract from the ballast low-molecular substances using the gel chromatography on
Sephadex G-100.
Based on the results of biological researches on the simulated generalized
candidamycosis of guinea pigs the high-molecular fraction was selected, which, in case
of the intradermal administration in the amount of 0.1 ml with the protein concentration
of 5 µg/ml in 24-48 hours causes the formation of erythema 18-22 mm and papula 5-
10 mm, i.e. Causes the delayed-type reaction.
BIBLIOGRAPHICAL REFERENCES
1.
Anane, S. (2007). Biological diagnosis of systemic candidiasis: difficulties
and future prospects. Pathologie Biologie, 55(5), 262–272.
2.
Horn, D. L., Neofytos, D. and Anaissie, E. J. (2009). Epidemiology and
outcomes of candidemia in 2009 patients: data from the prospective antifungal therapy
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